VR insert IV with 2 replicates #

Repeat 9/14/21 IVT with two sets of replicates to make sure results saw previously (or lack there of) are real. Used standard lab IVT protocol which is described in this spreadsheet table 9-21-21.

IVT results #

Ran IVT reactions out on gel for 2 hours at 60V TBE and then post stained with 400 ul of EtBr (wanted to go fast) for 20 mins before imaging. Did not destain.

Results look similar to previous IVT and replicates are also very similar which was nice to see.