Sat, Aug 28 21

VR vector digest with BglI(I) #

Today I came in to do a quick digest with what I thought was BglII on all inserts that came as a vector. I digested 100 ng of each insert with 0.1 ul of BglI (thinking it was BglII) and ran an undigested control along side it. The 0.8 TAE gel is below.

There were way more bands than I was expecting and so I double checked to make sure I ordered the correct enzyme because BglI looks really similar to BglII on a screen.

And of course I ordered the wrong one. Whomp whomp. Luckily the campus sci store stocks it so I will go purchase the correct enzyme and repeat my one pot PCR experiment Monday morning.