Received new IVT reagents #
No experiments today, but did get new reagents and buffers. All reagents in freezer box on my self in the kitchen -20C freezer.
Also started a reagents google sheet to record information when they arrive or begin use.
IVT reagent table #
| Reagent | Concentration | Recieved | Lot Number | Expiration Date | Location | Product Details |
|---|---|---|---|---|---|---|
| T7 RNA Polymerase | 50000 U/ml | 5/14/2021 | 10091329 | 11/23 | Ethan IVT Box -20 Fridge | www.neb.com/MO25S |
| 10X T7 RNA Polymerase Reaction Buffer | NA | 5/14/2021 | 10073286 | 7/23 | Ethan IVT Box -20 Fridge | www.neb.com/MO25S |
| T3 RNA Polymerase | 50000 U/ml | 5/14/2021 | 10085498 | 10/22 | Ethan IVT Box -20 Fridge | www.neb.com/MO378S |
| 10X T3 RNA Polymerase Reaction Buffer | NA | 5/14/2021 | 10073286 | 7/23 | Ethan IVT Box -20 Fridge | www.neb.com/MO378S |
| RNase H | 5000 U/ml | 5/14/2021 | 10081857 | 10/22 | Ethan IVT Box -20 Fridge | www.neb.com/MO297S |
| 10X RNase H Reaction Buffer | NA | 5/14/2021 | 10085445 | 10/23 | Ethan IVT Box -20 Fridge | www.neb.com/MO551S |
| E. coli RNA Polymerase Holoenzyme | 1000 U/ml | 5/14/2021 | 10088317 | 7/22 | Ethan IVT Box -20 Fridge | www.neb.com/MO551S |
| 5x E. coli Reaction Buffer | NA | 5/14/2021 | 10048086 | 7/22 | Ethan IVT Box -20 Fridge | www.neb.com/MO551S |
| rNTP Mix | 25 mM | 5/14/2021 | 10085968 | 10/22 | Ethan IVT Box -20 Fridge | www.neb.com/MO466S |