Thu, Apr 15 21

weight: 1 type: ‘docs’ #

IVT Plasmid pFC8 T1 T2 #

Hello there

Summary #

Redoing the IVT assay with different batch of the plasmid. This is a test.

Reagents #

NameVolume (ul)
5x Buffer4
100 mM DTT4
2.5 mM NTP1

Protocol Flow #

Note: When using more plasmids do not split the RNAse + and - cases until after the polymerase is added. Can keep these in one tube until then.

Results #

There is actually DNA this time but no band shifting :(