weight: 1 type: ‘docs’ #
IVT Plasmid pFC8 T1 T2 #
Hello there
Summary #
Redoing the IVT assay with different batch of the plasmid. This is a test.
Reagents #
| Name | Volume (ul) |
|---|---|
| 5x Buffer | 4 |
| 100 mM DTT | 4 |
| 2.5 mM NTP | 1 |
| DNA | 4 |
| H20 | 11 |
Protocol Flow #
Note: When using more plasmids do not split the RNAse + and - cases until after the polymerase is added. Can keep these in one tube until then.
Results #
There is actually DNA this time but no band shifting :(